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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2010 |
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Data da última atualização: |
07/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAPROTKA, T.; BOITEUX, L. S.; FONSECA, M. E. N.; RESENDE, R. O.; JESKE, H.; FARIA, J. C.; RIBEIRO, S. G. |
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Afiliação: |
T. PAPROTKA, UNIVERSITY OF STUTTGART; LEONARDO SILVA BOITEUX, CNPH; MARIA ESTHER DE N FONSECA BOITEUX, CNPH; R. O. RESENDE, UNB; H. JESKE, UNIVERSITY OF STUTTGART; JOSIAS CORREA DE FARIA, CNPAF; SIMONE DA GRACA RIBEIRO, CENARGEN. |
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Título: |
Genomic diversity of sweet potato geminiviruses in a Brazilian germplasm bank. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Virus Research, Amsterdam, v. 149, n. 2, p. 224-233, May 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Begomoviruses cause major diseases of sweet potato worldwide impairing considerably the yields of this important food staple. Since sweet potato plants are vegetatively propagated and globally transported, they are prone to accumulate and disseminate geminiviruses. Effective diagnostic tools are, therefore, desirable. We studied the genomic diversity of geminiviruses present in naturally-infected sweet potato accessions belonging to a Brazilian germplasm bank collection. Fifty-five samples from different sweet potato accessions displaying geminivirus-like symptoms were analyzed by combining rolling circle amplification (RCA) with restriction fragment length polymorphism (RFLP) and sequencing. The restriction enzyme MspI (HpaII) revealed diverse band patterns in 55 samples and digestion with BamHI, SstI or PstI resulted in full-length sweet potato geminivirus DNAs of about 3 kb in 46 samples. In addition, smaller fragments were identified as either viral ?Defective DNAs? (D-DNAs) or mitochondrial plasmid DNAs. The diversity of sweet potato-associated geminiviruses was found to be very high under Brazilian conditions. Representative viral full-length DNAs have been cloned and sequenced yielding two new tentative species, three strains and several variants of previously described sweet potato geminiviruses. Sequence comparisons identified footprints of recombination in their genomes underscoring the risk of generating new geminiviruses in vegetatively propagated germplasm bank material. The sites of recombination were found in conjunction with predicted hairpin structures. We propose diagnostic routines to screen germplasm bank material for geminiviruses by the rapid and reliable RCA/RFLP as the technique of choice. MenosBegomoviruses cause major diseases of sweet potato worldwide impairing considerably the yields of this important food staple. Since sweet potato plants are vegetatively propagated and globally transported, they are prone to accumulate and disseminate geminiviruses. Effective diagnostic tools are, therefore, desirable. We studied the genomic diversity of geminiviruses present in naturally-infected sweet potato accessions belonging to a Brazilian germplasm bank collection. Fifty-five samples from different sweet potato accessions displaying geminivirus-like symptoms were analyzed by combining rolling circle amplification (RCA) with restriction fragment length polymorphism (RFLP) and sequencing. The restriction enzyme MspI (HpaII) revealed diverse band patterns in 55 samples and digestion with BamHI, SstI or PstI resulted in full-length sweet potato geminivirus DNAs of about 3 kb in 46 samples. In addition, smaller fragments were identified as either viral ?Defective DNAs? (D-DNAs) or mitochondrial plasmid DNAs. The diversity of sweet potato-associated geminiviruses was found to be very high under Brazilian conditions. Representative viral full-length DNAs have been cloned and sequenced yielding two new tentative species, three strains and several variants of previously described sweet potato geminiviruses. Sequence comparisons identified footprints of recombination in their genomes underscoring the risk of generating new geminiviruses in vegetatively propagated germplasm bank ... Mostrar Tudo |
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Palavras-Chave: |
Batata-doce; Diversidade genética; Geminivírus. |
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Thesagro: |
Banco de germoplasma; DNA; Ipomoea Batatas. |
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Thesaurus Nal: |
Begomovirus; Gene banks; Genetic variation; Restriction fragment length polymorphism; Sweet potatoes. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02648naa a2200325 a 4500
001 1877070
005 2023-02-07
008 2010 bl uuuu u00u1 u #d
100 1 $aPAPROTKA, T.
245 $aGenomic diversity of sweet potato geminiviruses in a Brazilian germplasm bank.
260 $c2010
520 $aBegomoviruses cause major diseases of sweet potato worldwide impairing considerably the yields of this important food staple. Since sweet potato plants are vegetatively propagated and globally transported, they are prone to accumulate and disseminate geminiviruses. Effective diagnostic tools are, therefore, desirable. We studied the genomic diversity of geminiviruses present in naturally-infected sweet potato accessions belonging to a Brazilian germplasm bank collection. Fifty-five samples from different sweet potato accessions displaying geminivirus-like symptoms were analyzed by combining rolling circle amplification (RCA) with restriction fragment length polymorphism (RFLP) and sequencing. The restriction enzyme MspI (HpaII) revealed diverse band patterns in 55 samples and digestion with BamHI, SstI or PstI resulted in full-length sweet potato geminivirus DNAs of about 3 kb in 46 samples. In addition, smaller fragments were identified as either viral ?Defective DNAs? (D-DNAs) or mitochondrial plasmid DNAs. The diversity of sweet potato-associated geminiviruses was found to be very high under Brazilian conditions. Representative viral full-length DNAs have been cloned and sequenced yielding two new tentative species, three strains and several variants of previously described sweet potato geminiviruses. Sequence comparisons identified footprints of recombination in their genomes underscoring the risk of generating new geminiviruses in vegetatively propagated germplasm bank material. The sites of recombination were found in conjunction with predicted hairpin structures. We propose diagnostic routines to screen germplasm bank material for geminiviruses by the rapid and reliable RCA/RFLP as the technique of choice.
650 $aBegomovirus
650 $aGene banks
650 $aGenetic variation
650 $aRestriction fragment length polymorphism
650 $aSweet potatoes
650 $aBanco de germoplasma
650 $aDNA
650 $aIpomoea Batatas
653 $aBatata-doce
653 $aDiversidade genética
653 $aGeminivírus
700 1 $aBOITEUX, L. S.
700 1 $aFONSECA, M. E. N.
700 1 $aRESENDE, R. O.
700 1 $aJESKE, H.
700 1 $aFARIA, J. C.
700 1 $aRIBEIRO, S. G.
773 $tVirus Research, Amsterdam$gv. 149, n. 2, p. 224-233, May 2010.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
04/11/2014 |
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Data da última atualização: |
04/11/2014 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
BOGNOLA, I. A.; ANDRADE, G. de C.; MAEDA, S. |
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Afiliação: |
ITAMAR ANTONIO BOGNOLA, CNPF; GUILHERME DE CASTRO ANDRADE, CNPF; SHIZUO MAEDA, CNPF. |
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Título: |
Sustentabilidade de solos sob plantios florestais nos diversos biomas brasileiros. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: RIBEIRO, B. T.; WENDLING, B. (Ed.). Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas. Uberlândia: EDUFU, 2014. p. 49-61. |
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Idioma: |
Português |
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Palavras-Chave: |
Bioma; Brasil; Plantio florestal; Solos; Sustentabilidade. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00631naa a2200193 a 4500
001 1999161
005 2014-11-04
008 2014 bl uuuu u00u1 u #d
100 1 $aBOGNOLA, I. A.
245 $aSustentabilidade de solos sob plantios florestais nos diversos biomas brasileiros.
260 $c2014
653 $aBioma
653 $aBrasil
653 $aPlantio florestal
653 $aSolos
653 $aSustentabilidade
700 1 $aANDRADE, G. de C.
700 1 $aMAEDA, S.
773 $tIn: RIBEIRO, B. T.; WENDLING, B. (Ed.). Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas. Uberlândia: EDUFU, 2014. p. 49-61.
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Embrapa Florestas (CNPF) |
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